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Dried blood spots (DBS) can be used in creating nations to alleviate the logistic constraints of utilizing blood plasma specimens for viral load (VL) and HIV drug resistance (HIVDR) testing, however they needs to be assessed under area circumstances. Between 2009 and 2011, we collected paired plasma-DBS samples from therapy-experienced HIV-1-infected adults in Burkina Faso, Cameroon, Senegal, Togo, Thailand, and Vietnam. The DBS have been saved at an ambient temperature for two to 4 weeks and subsequently at ؊20°C earlier than testing. VL testing was performed on the plasma samples and DBS utilizing regionally accessible strategies: the Abbott m2000rt HIV-1 check, generic G2 real-time PCR, or the NucliSENS EasyQ model 1.2 take a look at. 1,000 copies/ml, HIVDR genotyping was carried out on paired plasma-DBS samples. Overall, we compared 382 plasma-DBS sample pairs for DBS VL testing accuracy. The sensitivities of the different assays in different laboratories for detecting VF using DBS varied from 75% to 100% for the m2000rt take a look at in labs B, C, and D, 91% to 93% for generic G2 realtime PCR in labs A and F, and 85% for the NucliSENS take a look at in lab E. The specificities diversified from 82% to 97% for the m2000rt and NucliSENS checks and reached only 60% for the generic G2 test. The NucliSENS take a look at showed good settlement between plasma and DBS VL however underestimated the DBS VL. The bottom agreement was noticed for the generic G2 take a look at. Genotyping was successful for 96/124 (77%) DBS tested, and 75/96 (78%) plasma-DBS pairs had identical HIVDR mutations. Significant discrepancies in resistance interpretations had been observed in 9 instances, 6 of which had been from the same laboratory. DBS may be successfully used instead to blood plasma samples for routine VL and HIVDR monitoring in African and Asian settings. However, the number of an enough VL measurement method and the definition of the VF threshold must be thought-about, and laboratory performance should be monitored.
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